Novus Biologicals™ MMP-9 Antibody Pair [HRP]

Description

Polyclonal/Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes an antibody specific for Rat MMP-9.

Specifications

• Assay Range: 19.53 pg/mL
• Assay Sensitivity: 19.53-1250 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes an antibody specific for Rat MMP-9.
• Target Species: Rat
• For Use With (Application): Sandwich ELISA
• Gene Alias: 92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, EC 3.4.24, EC 3.4.24.35, Gelatinase B, GELB, macrophage gelatinase, MANDP2, matrix metallopeptidase 9, matrix metalloproteinase 9, matrix metalloproteinase-9, MMP-9, type V collagenase
• Gene ID (Entrez): 4318
• Gene Symbol: MMP9
• Host Species: Rabbit/Mouse
• Kit Contents:
  Standard: Each vial contains 75 ng of recombinant Rat MMP-9.
  Capture Antibody: 0.5 mg/mL of mouse anti-Rat MMP-9 monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.2 mg/mL of rabbit anti-MMP-9 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Angiogenesis, Cancer, Cellular Markers, Extracellular Matrix, GPCR
• Storage Requirements: Storage is content dependent.
• Target: MMP-9
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1250 pg/mL is recommended.