Invitrogen™ Human MMP3 ELISA Kit

Catalog No.	Quantity
BMS20143	96 Tests
BMS20143TEN	10 x 96 Tests

Catalog No. BMS20143 Supplier Invitrogen™ Supplier No. BMS20143

Description

ELISA

The Human Matrix MetalloProteinase 3 (Hu MMP3) ELISA quantitates Hu MMP3 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MMP3. Principle of the method The Human MMP3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

MMP3 (stromelysin-1) belongs to the peptidase M10A subfamily. It is secreted in its pro-protein form, and is activated by extracellular proteinase cleavage. MMP3 is capable of binding to zinc and calcium ions, and has metallo-endopeptidase activity. The active enzyme degrades collagen, gelatin, fibronectin, and laminin. It is involved in normal cellular processes such as wound repair and tissue remodeling. Mutations in the gene can result in coronary heart disease 6.

Specifications

• Accession Number: P08254
• Assay Range: 0.06 to 4.00 ng/mL
• Assay Sensitivity: 0.0005 ng/ml
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 4314
• Gene Symbol: CHDS6,MMP-3,SL-1,STMY,STMY1,STR1
• Interassay CV: 4%
• Intraassay CV: 6.1
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
• Storage Requirements: 2°C to 8°C
• Target: MMP3
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs 30 mins