Invitrogen™ Human MMP-9 Recombinant Protein

Catalog No.	Quantity
PIRP75654	100 μg

Catalog No. PIRP75654 Supplier Invitrogen™ Supplier No. RP75654

Description

Recombinant Protein

This product contains purified recombinant human MMP-9 protein with an N-terminal hexahistidine tag. The recombinant protein is a fragment corresponding to the catalytic domain of the protein (residues 113-450). This product may be used as a positive control in many immunological assays.

MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. MMP9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. Further, MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Studies have shown that elevated MMP9 is associated with progression of idiopathic atrial fibrillation and aortic aneurysm.

Specifications

• Accession Number: P14780
• Concentration: 0.27 mg/mL
• For Use With (Application): Control, Western Blot
• Formulation: 20 mM tris HCl with 50% glycerol and no preservative; pH 8.0
• Gene ID (Entrez): 4318
• Molecular Weight (g/mol): 42.03 kDa
• Name: Human MMP-9
• pH Range: 8
• Quantity: 100 μg
• Source: E. Coli
• Regulatory Status: RUO
• Gene Alias: 67 kDa matrix metalloproteinase-9; 82 kDa matrix metalloproteinase-9; 92 kDa gelatinase; 92 kDa type IV collagenase; 92 kD gelatinase; 92 kD type IV collagenase; 92 kDa gelatinase; 92 kDa type IV collagenase; 92-kDa type IV collagenase; AW743869; B/MMP9; Clg4b; collagenase type IVB; fj05a08; Gel B; gelatinase B; Gelatinase-B; GELB; macrophage gelatinase; MANDP2; matrix metallo protease; matrix metallopeptidase 9; matrix metallopeptidase 9 (gelatinase B, 92 kDa gelatinase, 92 kDa type IV collagenase); matrix metalloproteinase 9; matrix metalloproteinase 9 (gelatinase B 92-kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92 kDa gelatinase, 92 kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92 kDa matrix metalloproteinase 9 (gelatinase B, 92 kDa gelatinase, 92 kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92-kDa type IV collagenase); Matrix metalloproteinase9; matrix metalloproteinase-9; MMP; Mmp9; MMP-9; mmp9 protein; MMP-9 protein; MMPs; Preproform of 92-kDa type IV collagenase (MMP-9): N-terminal; pro-MMP-9; type IV collagenase; type V collagenase; wu:fb02g06; wu:fb07b05; wu:fi98c09; wu:fj05a08; ZFMMP-9; zgc:64165
• Common Name: MMP-9
• Gene Symbol: MMP9
• Conjugate: Unconjugated
• Species: E. coli
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Amino acids Asp113- Glu450 containing an N-terminal His-tag
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Protein Subtype: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov