Learn More
Invitrogen™ Carboxy-H2DCFDA (general oxidative stress indicator)
Description
General oxidative stress indicator
6-carboxy-2',7'-dichlorodihydrofluorescein diacetate is a chemically reduced, acetylated form of fluorescein used as an indicator for reactive oxygen species (ROS) in cells. This nonfluorescent molecule is readily converted to a green-fluorescent form when the acetate groups are removed by intracellular esterases and oxidation (by the activity of ROS) occurs within the cell. We offer the carboxylated H2DCFDA analog (carboxy-H2DCFDA, Cat. No. C400), which has two negative charges at physiological pH, and its di(acetoxymethyl ester) (Cat. No. C2938). Upon cleavage of the acetate and ester groups by intracellular esterases and oxidation, both analogs form carboxydichlorofluorescein (Cat. No. C368), with additional negative charges that impede its leakage out of the cell.
ROS Indicator Specifications:
- Ex/Em: ∼492–495/517–527 nm
- Product is air sensitive and should be stored under dry argon or nitrogen
- Product may be dissolved in DMSO, DMF, or ethanol for use
- Indicator is cell permeant (cell loading protocols are available in the literature)
- Fluorescence can be monitored using a flow cytometer, fluorometer, microplate reader, or fluorescence microscope, using excitation sources and filters appropriate for fluorescein
Find More ROS Indicators
We offer an assortment of Molecular Probes™ products for the generation of reactive oxygen species (ROS), including singlet oxygen, superoxide, hydroxyl radical and various peroxide and hydroperoxides, as well as for their fluorometric detection in solution. Review Generating and Detecting Reactive Oxygen Species—Section 18.2 in the Molecular Probes™ Handbook for more information on these products.
For Research Use. Not for human or animal therapeutic or diagnostic use.
Order Info
Shipping Condition: Room Temperature
Specifications
Specifications
| Quantity | 25 mg |
| For Use With (Application) | ROS |
| Detection Method | Fluorescence |
| Product Type | ROS Indicator |
| Excitation Wavelength Range | 492 to 495 nm |
| Emission Wavelength Range | 517 to 527 nm |
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.