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Invitrogen™ Cholera Toxin Subunit B (Recombinant), Alexa Fluor™ 555 Conjugate

Made from a recombinant version of the B subunit only to provide a very high-purity product that is completely free of the toxic A subunit

Supplier: Invitrogen™ C22843

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Catalog No. C22843

$920.00 / Each of 1

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Description

Cholera toxin B subunit (CT-B) attaches to cells by binding to ganglioside G_M1, making it powerful tool for retrograde labeling of neurons
This tracer has been used in variety of applications, including tracing of rat forebrain afferents, projections of the parabrachial region, and neurons of the urinary bladder wall
When used in neuronal tracing applications, CT-B is typically introduced by pressure injection or by iontophoretic injection into neural tissue
At neutral pH, 11.4 kDa B subunit exists as 57 kDa pentamer
Lyophilized product can be dissolved in buffer (e.g., PBS) for use
More recently, researchers have found that CT-B can be used as marker for lipid rafts, which are membrane microdomains enriched in cholesterol and sphingolipids thought to be important in cell signaling
For lipid raft staining, cells are first incubated with fluorescent CT-B, then an anti-CT-B antibody is added to crosslink the CT-B in the lipid rafts into distinct patches on the plasma membrane
These patches are easily visualized by fluorescence microscopy

Label (Ex/Em): Alexa Fluor™ 555 (555/565nm)
At neutral pH, the 11.4 kDa B subunit exists as a 57 kDa pentamer

Cell Analysis, Cell Structure, Cell Tracing and Tracking, Cell Viability, Proliferation and Function, Cellular Imaging, Endocytosis, Exocytosis and Phagocytosis, Immunofluorescence (IF), Immunofluorescence Staining and Detection, Lipid Rafts, Microbial Tracking, Neuronal Tracing

Order Info

Shipping Condition: Room temperature

Specifications

Quantity	500μg
Recombinant	Recombinant
Label Type	Alexa Fluor Dyes
Protein Form	Recombinant
Protein Subtype	Cholera Toxin

Conjugate	Alexa Fluor 555
Content And Storage	Store in freezer (-5 to -30°C) and protect from light.
Shipping Condition	Room Temperature
Form	Lyophilized
Product Line	Alexa Fluor™

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