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Invitrogen™ DAF-FM Diacetate (4-Amino-5-Methylamino-2',7'-Difluorofluorescein Diacetate)

For detection and quantification of low concentrations of nitric oxide (NO)

$427.00 - $710.00


Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
D23844
Encompass
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Invitrogen™
D23844
10 x 50 μg
Each for $427.00
Add to cart
 
D23842
Encompass
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Invitrogen™
D23842
1 mg
Each for $710.00
Add to cart
 
Description

Description

It is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. DAF-FM fluorescence can be detected by any instrument that can detect fluorescein, including flow cytometers, microscopes, fluorescent microplate readers and fluorometers.

  • Ex/Em of DAF-FM: approx. 495/515nm
  • Lyophilized product should be dissolved using DMSO and then added to an aqueous buffer to create a working solution
  • DAF-FM diacetate is cell permeant and passively diffuses across cellular membranes; once inside the cell, it is converted to cell-impermeant for
  • Buffers containing bovine serum albumin (BSA) and phenol red may affect fluorescence and should be used with caution
  • Fluorescence quantum yield of DAF-FM is approx. 0.005, but increases about 160-fold, to ∼0.81, after reacting with NO
  • Assessment of NO production in transaldolase-deficient lymphoblasts by flow cytometry
  • Detection of NO accumulation in embryonic cortical neurons following neurotrophin stimulation
  • in vivo imaging of NO in zebrafish
I

  • ntravital microscopic detection of NO generation associated with angiogenesis in mice
  • Quantitation of ATP-induced NO release in rabbit platelets
  • Spectra of the NO adduct of DAF-FM are independent of pH above pH 5.5
  • NO adduct of DAF-FM is significantly more photostable than that of DAF-2, which means additional time for image capture
  • DAF-FM is a more sensitive reagent for NO than is DAF-2 (NO detection limit for DAF-FM approx. 3nM versus approx. 5nM for DAF-2)

Refer to the Molecular Probes™ Handbook for additional product information.

Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Nitric Oxide Research, Nitro-Oxidative Stress

Order Info

Shipping Condition: Room Temperature

SDS
Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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