Invitrogen™ FilmTracer™ FM™ 1-43 Green Biofilm Cell Stain

Catalog No.	Quantity
F10317	1 mg

Catalog No. F10317 Supplier Invitrogen™ Supplier No. F10317

Description

Lipophilic styryl compounds suitable for wide variety of studies involving plasma membrane and vesiculation

The water-soluble FM dyes are virtually nonfluorescent in aqueous media and are believed to insert into the surface membrane where they become intensely fluorescent. FM 1-43 stain has been used successfully to stain the cell bodies specifically in a complex biofilm mileu, including Pseudomonas aeruginosa, Escherichia coli, Staphylococcus sp., Acidothiobacillus caldus, and Vibrio cholerae.

• Flow Cytometer Laser Lines: 488
• Platform: Confocal Microscope, Flow Cytometer, Fluorescence Microscope, Fluorescent Imager, Microplate Reader
• Label or Dye: FM 1-43/lipid

Analysis of Bacteria, Bacterial Staining & Detection, Bacterial Tracing/Tracking/Morphology, Cell Analysis, Microbiological Analysis

Order Info

Shipping Condition: Room Temperature

Specifications

• Color: Green
• Content And Storage: Store at room temperature; Dessicate; Protect from light
• Excitation Wavelength Range: 472⁄580
• Dye Type: FM 1-43⁄lipid
• For Use With (Equipment): Confocal Microscope, Fluorescence Microscope, Flow Cytometer, Microplate Reader, Fluorescent Imager
• Quantity: 1 mg
• Detection Method: Fluorescence
• Emission: Visible
• Form: Solid
• Product Line: FilmTracer, Molecular Probes
• Shipping Condition: Room Temperature
• Label Type: Other Labels or Dyes
• Product Type: Dye
• Sub Cellular Localization: Cell Membranes, Lipids

Frequently Asked Questions (FAQs)

The product sheet for FM 1-43 Dye states that the maximum for excitation is 510 nm and emissions is 626 nm, but the spectrum on the product page shows a peak at 470 nm and 580 nm. Can you clarify what the excitation and emission maxima is for this dye?

FM lipophilic styryl dyes are known to shift their spectral properties upon binding to membranes. These dyes exhibit an appreciable solvent and environmental dependency in both free and membrane-bound forms. The emissions of the membrane-bound dye shifts to a shorter wavelength compared to the emission in the solvent.

In solvent, FM 1-43 Dye (Cat. Nos. T35356, T3163, F35355, F10317) absorbs in the range of fluorescein (~490 nm) and initially emits at around 626-636 nm. However, upon membrane integration, it exhibits emission in the 565-590 nm range. Because this dye exhibits a large Stokes shift, it does not perfectly fit into a green or orange fluorophore profile, but is rather a yellow-fluorescent dye, and may require a long-pass emission filter.

Note: FM 1-43 Dye is often used with typical fluorescein or GFP optical filter sets for biological imaging but is poorly excited through tetramethylrhodamine filters.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.