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Invitrogen™ FluoCells™ Prepared Slide #1 (BPAE cells with MitoTracker™ Red CMXRos, Alexa Fluor™ 488 Phalloidin, and DAPI)

Catalog No. f36924
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Catalog No. F36924 Supplier Invitrogen™ Supplier No. F36924
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Description

Contains fixed, multi-labeled, cell preparations for observation by fluorescence microscopy validated for immunofluorescence

FluoCells Prepared Slide #1 contains bovine pulmonary artery endothelial cells (BPAEC). MitoTracker Red CMXRos was used to stain the mitochondria in the live cells, with accumulation dependent upon membrane potential. Following fixation and permeabilization, F-actin was stained with Alexa Fluor 488 phalloidin, and the nuclei were counterstained with the blue-fluorescent DNA stain DAPI.

Cell Analysis, Cellular Imaging, Immunofluorescence (IF)

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Shipping Condition: Room temperature

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Specifications

Type Prepared Microscope Slide
Product Line Alexa Fluor FluoCells MitoTracker
For Use With (Application) Immunofluorescence
Content And Storage Store at room temperature and protect from light.
Quantity 1 Ea.
Shipping Condition Room Temperature

Frequently Asked Questions (FAQs)

Do you have any slides of pre-labeled cells or tissues that I can use to demonstrate with?

Some of the most popular are the FluoCells demonstration slides, which have cells or tissue sections that are labeled with three different dyes. FluoCells 1 and 2 have cultured cells, while FluoCells 3 is a tissue section (mouse kidney). Slides with fluorescent beads are also available.
DAPI and Hoechst dyes are quite similar to each other. Why would I choose one over the other?

DAPI is a very common blue-fluorescent dye for nuclear counterstaining and gives very bright labeling on nuclei in fixed and permeabilized cells and tissues. However, it is considered to be a semi-permeant to impermeant stain and provides inconsistent staining of live cells. Hoechst 33342 dye is cell-permeant and stains with the same binding mechanism and fluorescent color; it is preferred for live-cell imaging and is just as good as DAPI for fixed cell labeling.

For Research Use Only. Not for use in diagnostic procedures.

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