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Invitrogen™ FM™ 4-64FX, fixable analog of FM™ 4-64 membrane stain

Catalog No. F34653
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Catalog No. F34653 Supplier Invitrogen™ Supplier No. F34653
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Description

Fixable analog of FM 4-64 membrane stain

The lipophilic probe, FM 4-64FX exhibits low fluorescence in water but fluoresces intensely upon binding the outer leaf of the plasma membrane providing discrete plasma membrane staining. The binding is rapid and reversible, and “FX” denotes that this analog is fixable with aldehyde-based fixatives. It is also an excellent reagent for identifying actively firing neurons and for investigating the mechanisms of activity-dependent vesicle cycling.

Cell Analysis, Cell Structure, Cell Tracing & Tracking, Cell Viability, Proliferation & Function, Endocytosis, Exocytosis & Phagocytosis, General Cell Tracing, Microbial Tracking, Neuronal Tracing, Plasma Membrane

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Color Infrared
Content And Storage Store at room temperature and protect from light.
For Use With (Equipment) Fluorescence Microscope
Quantity 10 x 100 μg
Detection Method Fluorescence
Product Line FM
Shipping Condition Room Temperature
Label Type Fluorescent Dye
Product Type Lipophilic Probe
Sub Cellular Localization Plasma Membrane

Frequently Asked Questions (FAQs)

I want to study endosomes trafficking using FM 4-64. Will the label be retained after fixation? And can I label already-fixed cells?

No. For that you would need the FM 4-64FX version. The non-FX version will not be retained upon fixation, leading to loss of much of the stain and an increase in background. The FX version will be retained using an aldehyde-based fixative. Cells that are already fixed will be stained throughout the cell and the signal will not be localized; it is recommended to stain live cells and then fix.
I want to study endosome trafficking using FM 4-64 dye. Will the label be retained after fixation? And can I label cells that have already been fixed?

No. For that, you would need the FM 4-64FX version. The non-FX version will be lost, leading to loss of much of the specific label and a vast increase in background labeling. The FX version will be fixed in place with formaldehyde. Cells that have been fixed already will not label correctly, so you will need to label the cells live and then fix.

For Research Use Only. Not for use in diagnostic procedures.

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