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Invitrogen™ HPF (Hydroxyl Radical and Peroxynitrite Sensor)

Catalog No. H36004
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H36004 470 μL
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Catalog No. H36004 Supplier Invitrogen™ Supplier No. H36004
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Description

Two indicators that together can selectively detect hypochlorite anion

Aminophenyl fluorescein (APF, A36003) and hydroxyphenyl fluorescein (HPF), two reactive oxygen species (ROS) indicators developed by T. Nagano, offer greater specificity and stability than dichlorodihydrofluorescein diacetate (H2DCFDA, D399). APF and HPF show limited non-selective reactivity and relatively high resistance to light-induced oxidation. Both of these new fluorescein derivatives are nonfluorescent until they react with the hydroxyl radical or peroxynitrite anion; APF will also react with the hypochlorite anion. Together, these two ROS indicators can selectively detect hypochlorite anion. Upon oxidation, both APF and HPF exhibit bright green fluorescence (excitation/ emission maxima ∼490/515 nm), making them compatible with any instrument that can detect fluorescein.

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Shipping Condition: Room Temperature

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Specifications

Quantity 470 μL
For Use With (Application) ROS
Detection Method Fluorescence
Product Type ROS Indicator
Excitation Wavelength Range 490 nm
Emission Wavelength Range 515 nm

For Research Use Only. Not for use in diagnostic procedures.

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