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Invitrogen™ HCS LipidTOX™ Green Neutral Lipid Stain, for cellular imaging
Description
The intracellular accumulation of neutral lipids, steatosis, is often triggered by drugs that affect the metabolism of fatty acids and/or neutral lipids. HCS LipidTOX Green neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX phospholipidosis detection reagents. HCS LipidTOX neutral lipid stains can also be used to monitor the formation and differentiation of adipocytes, a process called adipogenesis.
Cell Analysis, Cellular Imaging, High-Content Screening (HCS)
Order Info
Shipping Condition: Room temperature
Specifications
Specifications
| Color | Green |
| Content And Storage | Store in freezer -5°C to -30°C and protect from light. |
| For Use With (Equipment) | High Content Instrument |
| Quantity | 1 each |
| Detection Method | Fluorescence |
| Product Line | LipidTOX |
| Shipping Condition | Room Temperature |
| Label Type | Fluorescent Dye |
| Product Type | Lipid Stain |
| Sub Cellular Localization | Cell Membranes, Lipids |
Frequently Asked Questions (FAQs)
LipidTOX Green neutral lipid stain can be imaged with filter sets appropriate for Alexa Fluor 488 dye or fluorescein. LipidTOX Red neutral lipid stain is best imaged with filter sets appropriate for Alexa Fluor 594 dye or Texas Red dye. LipidTOX Deep Red neutral lipid stain can imaged with filter sets appropriate for Alexa Fluor 647 dye or Cy5 dye.
For live-cell imaging, the CellVue and CellMask Plasma Membrane Stains are the most uniform and the slowest to be endocytosed. However, they are not the best choice if you wish to fix and permeabilize your cells, such as for antibody labeling. Wheat germ agglutinin (WGA) conjugates are also able to label live cells, or can label already formaldehyde-fixed cells. They can survive subsequent permeabilization with detergents, such as Triton X-100. If cells are already permeabilized, WGA will label internal structures as well. Thus, only an antibody against a plasma membrane protein can be used if cells are already permeabilized. Lipophilic cyanine dyes, such as DiI, will label all cell membranes in live cells, not just plasma membranes, if left on live cells for extended periods. Following page will help you choose (http://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-structure/plasma-membrane.html).
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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