Invitrogen™ SYTOX™ Green Nucleic Acid Stain - 5 mM Solution in DMSO

Catalog No.	Quantity
S7020	250 μL

Catalog No. S7020 Supplier Invitrogen™ Supplier No. S7020

Description

Useful indicator of dead cells within a population

SYTOX Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells.

Simple Viability Determination
SYTOX Green allows quick determination of cell viability when using flow cytometers, fluorescence microscopes, fluorometers, or fluorescence microplate readers. It will not cross intact membranes, but will easily penetrate compromised membranes characteristic of dead cells. Because it exhibits >500-fold fluorescence enhancement upon binding nucleic acids, briefly incubating dead cells with SYTOX Green will result in bright green fluorescence with an emission peak of 523nm when excited by a 488nm argon-ion laser or any other 450–490nm source. The exceptional brightness of the signal produced in dead cells makes SYTOX Green particularly useful for determining viability of not just mammalian cells, but both Gram-positive and Gram-negative bacteria.

Part of the Invitrogen Family of Cell Viability Stains and Assays
SYTOX stains for dead cells are available in a variety of colors, including red, blue, and orange. SYTOX stains have been incorporated into a number of assays for apoptosis, cell viability, and metabolism. 5mM solution in DMSO.

• Impermeant to live cells
• Excitation/Emission: 504/523nm
• Use with 488 Argon-ion laser
• Works with mammalian cells and both Gram-positive and Gram-negative bacteria

Cell Adhesion, Cell Analysis, Cell Cycle, Cell Structure, Cell Tracing and Tracking, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function, Cellular Imaging, Flow Cytometry, Flow Cytometry Cell Cycle Assays, Flow Cytometry Staining by Cell Structure, Flow Cytometry Viability and Cytotoxicity Assays, Flow Cytometry of Cellular Processes, Immunofluorescence (IF), Immunofluorescence Counterstaining, Mounting and Fade Prevention, Immunofluorescence Staining and Detection, Microbial Tracking, Nucleus, Nucleoli and Nuclear Envelope, Organelle Tracing

Order Info

Shipping Condition: Room Temperature

Specifications

• Color: Green
• Content And Storage: Store in freezer at -5°C to -30°C and protect from light.
• Excitation Wavelength Range: 504 nm
• Dye Type: Cell-Permeant
• Format: Tube(s)
• For Use With (Equipment): Flow Cytometer
• Quantity: 250 μL
• Volume (Metric): 250 μL
• Detection Method: Fluorescence
• Emission: 523 nm
• Form: Solution
• Product Line: SYTOX
• Shipping Condition: Room Temperature
• Label Type: Fluorescent Dye
• Product Type: Nucleic Acid Stain
• SubCellular Localization: Nucleic Acids, Nucleus

Frequently Asked Questions (FAQs)

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

I need to mount my Qdot secondary-labeled tissue samples in HistoMount mounting medium, but my Qdot 705 conjugate overlaps with your Qnuclear Deep Red nuclear label. What other nuclear label would you recommend, that is compatible with HistoMount mounting medium?

We recommend using SYTOX Green stain, which you can image using a FITC filter and we have shown to be compatible with HistoMount mounting medium. Some have used DAPI, but there have been some issues with slight quenching and spectral shifting of DAPI into green or even red wavelengths.

I am using SYTOX AAdvanced as a dead cell stain, but all of my cells are labeling even though I am certain that they are supposed to be alive. These are adherent cells that I have trypsinized. Why am I getting false-dead signals?

SYTOX AAdvanced labels only dead cells because it is a cell impermeant dye. The dye can only enter cells that have a compromised plasma membrane. Trypsinization may cause temporary disruption of the plasma membrane, sufficient to allow staining with a cell impermeant dye. You can reduce the “false-dead” problem by either reducing the amount of trypsin and/or reduce the incubation time for trypsinization or use a gentler dissociation reagent such as TrypLE Express, TrypLESelect reagents, or Versene. After trypsinization, wash well, and if possible, allow a recovery time in normal culture media before staining with any of the SYTOX dyes.

For Research Use Only. Not for use in diagnostic procedures.