Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ Molecular Probes™ Tali™ Apoptosis Kit - Annexin V Alexa Fluor 488 & Propidium Iodide Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Discrimination of apoptotic from necrotic and live cells in a population

Manufacturer:  Invitrogen™ A10788

Catalog No. A10788


Add to cart

Includes: Annexin V – Alexa Fluor 488 conjugate, Annexin Binding solution, Propidium Iodide (PI) solution

Description

Description

The TaliApoptosis Kit enables identification of apoptotic cells. The kit stains apoptotic cells with green Annexin V – Alexa Fluor™ 488, stains necrotic cells with both red propidium iodide and green Annexin V – Alexa Fluor 488, and does not stain live cells.

Apoptosis is a carefully regulated process of cell death that occurs as a normal part of development. Inappropriately regulated apoptosis is implicated in disease states, such as Alzheimer's disease and cancer. Apoptosis is distinguished from necrosis by characteristic morphological and biochemical changes, including compaction and fragmentation of the nuclear chromatin, shrinkage of the cytoplasm, and loss of membrane asymmetry.

Easily Discriminate Apoptotic from Necrotic Cells with Two-color Staining
In normal live cells, phosphatidyl serine (PS) is located on the cytoplasmic surface of the cell membrane. In apoptotic cells, however, PS is translocated from the inner to the outer surface of the plasma membrane, exposing it to the extracellular environment. The human anticoagulant Annexin V displays a high affinity for PS. Annexin V labeled with a fluorophore such as Alexa Fluor 488 can be used to identify apoptotic cells by binding to the exposed PS.

The TaliApoptosis Kit includes a solution of propidium Iodide (PI). PI is a cell-impermeant fluorogenic DNA-binding dye used for years to identify necrotic cells. PI is impermeant to live cells, but easily enters dead cells where it binds to nucleic acids and becomes fluorescent.

After staining a cell population using the TaliApoptosis Assay Kit with Annexin V-Alexa Fluor 488 and Propidium Iodide, apoptotic cells display green fluorescence, dead cells display red and green fluorescence and live cells show little or no fluorescence.

Annexin V Translocation, Apoptosis, Cell Analysis, Cell Counting and Viability, Cell Counting, Viability, and Cryopreservation, Cell Culture, Cell Viability, Proliferation and Function, Cellular Imaging, Imaging Cytometry

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Order Info

Shipping Condition: Wet ice

Specifications

Specifications

Tali - Annexin V Alexa Fluor™ 488, Tali - Propidium Iodide, Tali - Annexin V Binding Buffer
Annexin V Translocation, Apoptosis, Cell Analysis, Cell Counting and Viability, Cell Counting, Viability, and Cryopreservation, Cell Culture, Cell Viability, Proliferation and Function, Cellular Imaging, Imaging Cytometry
1 kit
Store Tali Annexin V Alexa Fluor 488 at 2 – 6°C. Store Tali Propidium Iodide at 2 – 6°C. Store Tali Annexin V Binding Buffer at 2 – 6°C.
Documents
Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only. Not for use in diagnostic procedures.