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Invitrogen™ Tubulin Tracker™ Green (Oregon Green™ 488 Taxol, Bis-Acetate), for live-cell imaging

Catalog No. T34075
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Catalog No. T34075 Supplier Invitrogen™ Supplier No. T34075
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Description

For live-cell imaging

Tubulin Tracker Green (Oregon Green 488 Taxol, bis-acetate) provides green-fluorescent staining of polymerized tubulin in live cells. Tubulin Tracker Green is an uncharged, nonfluorescent compound that easily passes through the plasma membrane of live cells. Once inside the cell, the lipophilic blocking group is cleaved by non-specific esterases, resulting in a green-fluorescent, charged form.

Cell Analysis, Cell Structure, Cell Tracing & Tracking, Cytoskeleton, Organelle Tracing

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store in freezer -5°C to -30°C and protect from light.
Dye Type Oregon Green™ 488
Quantity 1 set
Product Line Oregon Green
Shipping Condition Room Temperature
Label Type Classic Dyes
Product Type Tubulin Tracker
Sub Cellular Localization Cytoskeleton, Tubulin

Frequently Asked Questions (FAQs)

What is the difference between Tubulin Tracker Green (Cat No. T34078) and Tubulin Tracker Green, for live cell imaging (Cat. No. T34075)?

Both products include the same Tubulin Tracker Green reagent. The difference is in the packaging and amount of Tubulin Tracker Green reagent contained. Tubulin Tracker Green, for live cell imaging (Cat. No. T34075) includes enough Tubulin Tracker Green reagent for 300 slides whereas Tubulin Tracker Green (Cat No. T34078) contains enough Tubulin Tracker Green reagent for 60 slides. Also, Tubulin Tracker Green, for live cell imaging (Cat. No. T34075) does not include Probenecid.
With Tubulin Tracker Deep Red and Tubulin Tracker Green, can I fix samples after labeling?

No. The labeling is not retained upon fixation. Imaging of the samples should be done only on live cells. To limit photobleaching while viewing live cells, use ProLong Live Antifade Reagent, for live cell imaging (Cat No. P36974 and P36975).

How is Docetaxel in Tubulin Tracker Deep Red different from Taxol in Tubulin Tracker Green? Are there any differences in the binding or cytotoxicity between the two reagents?

Docetaxel has a slightly higher binding affinity than Taxol and exhibits longer retention time due to better uptake and slower efflux, but for use in labeling cultured cells, these differences should be minimal. More information comparing the properties of these two reagents is provided in this publication: https://www.ncbi.nlm.nih.gov/pubmed/1671606 https://www.cell.com/fulltext/S1074-5521(05)00301-7

For Research Use Only. Not for use in diagnostic procedures.

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