MilliporeSigma™ Mouse anti-acetyl-alpha tubulin, Clone: 6-11B-1,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABT868

Catalog No. MABT868MI

Description

Specifically detects acetyl-alpha tubulin clone: 6-11B-1 in Bovine, Drosophila, Human, Monkey, Mouse, Sea Urchin samples, and it is validated for Dot Blot, Electron Microscopy

Microtubules are cytoskeletal filaments composed of of alpha-/beta-tubulin heterodimers that self-assemble head-to-tail to form protofilaments and laterally to form a hollow tube. Various post-translational modifications (PTMs), including acetylation, polyglutamylation, polyglycylation, detyrosination, phosphorylation, and palmitoylation, can regulate the polymerization properties of tubulins and/or their interactions with microtubule associated proteins (MAPs) and motor proteins. A subset of cytoplasmic microtubules and microtubules in the spindle, axon, and cilia are known to contain alpha-tubulin acetylated via the epsilon-amino group of Lysine-40 (K40). The GNAT lysine acetyltransferase family member MEC-17 (or alpha-tubulin acetyltransferase/alphaTAT) catalyzes K40 acetylation, while HDAC6 and sirtuin2 (SIRT2) are known to catalyze tubulin deacetylation. Experimental evidences indicate that K40 acetylation primes alpha-tubulin for further PTM(s), which cannot be reversed by later deactylation of K40ac. Subsequently, the acetylated and deacetylated alpha-tubulin in native microtubules are structurally distinct from that of unacetylated alpha-tubulin (never before has its K40 acetylated).

Specifications

• Antigen: acetyl-alpha tubulin
• Classification: Monoclonal
• Formulation: Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Symbols: acetyl-alpha tubulin
• Immunogen: 15 S dynein fraction from sea urchin sperm axoneme.
• Quantity: 200 μg
• Research Discipline: Cell Structure
• Gene ID (Entrez): AEI99579.1
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
• Isotype: IgG2b κ

• Applications: Dot Blot, Immunoassay
• Clone: 6-11B-1
• Gene Accession No.: P18258
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Bovine, Drosophila, Human, Monkey, Mouse, Sea urchin
• Form: Purified

For Research Use Only