Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

MilliporeSigma™ Mouse anti-Active-β-Catenin, Clone: 8E7, Alexa Fluor 555,

Mouse Monoclonal Antibody

Manufacturer:  MilliporeSigma™ 05665AF555

Catalog No. 05-665-AF555


Add to cart

Description

Description

Specifically detects Active-β-Catenin clone: 8E7 in Human, Mouse samples, and it is validated for Immunocytochemistry, Immunohistochemistry

Catenin beta-1 (UniProt P35222; also known as beta-Catenin) is encoded by the CTNNB1 (also known as CTNNB, MRD19) gene (ORF OK/SW-cl.35, PRO2286; Gene ID 1499) in human. beta-Catenin is a multifunctional protein involved in the regulation of cell–cell adhesion and in the control of the Wnt signaling pathway. beta-Catenin contains in its central region 12 armadillo repeats that mediate its interaction with a variety of proteins including axin, adenomatous polyposis coli (APC), and T cell factor (TCF) family transcription factors. The C-terminal domain (CTD) of beta-catenin is implicated in transcriptional functions, while the N-terminal domain (NTD) contains several posttranslational modification (ubiquitination, acetylation, and phosphorylation) sites, including the Ser33/Ser37/Thr41 residues, important for beta-catenin stability regulation. In the absence of Wnt pathway activation, beta-catenin is retained in the cytoplasm in a 'destruction complex' with Axin, APC, and GSK3beta, where it is constitutively destined for proteasomal degradation as a result of constant phosphorylation by GSK3beta. Activation of the Wnt pathway results in stabilization and nuclear translocation of beta-catenin, resulting in beta-catenin target genes transcription. In addition to GSK3beta, PKCalpha is also reported to phosphorylate the Ser33/37/Thr41 residues of beta-catenin, and beta-catenin Ser45 is a known phosphorylation site for CK1alpha. In addition, HDAC6 and p300/CBP-associated factor (PCAF) regulate beta-catenin Lys49 acetylation.
Specifications

Specifications

Active-β-Catenin
8E7
Purified mouse conjugate antibody in PBS with 15mg/mL BSA and 0.1% sodium azide.
Purified
Murine
IgG1, κ
100μL
Cell Structure
Primary
NP_001895
Immunocytochemistry, Immunohistochemistry
Alexa Fluor 555
P35222
CTNNB1; CTNNB; PRO2286
Fusion protein corresponding to residues 1-100 of human β-catenin with a c-terminal histidine tag.
Protein G Purified
RUO
Stable for one year at 2°C to 8°C from date of receipt.
Monoclonal
Human, Murine
Documents
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only