CD3 Mouse anti-Human, APC-R700, Clone: UCHT1, BD

Catalog No.	Quantity
BDB565120	25 Tests
BDB565119	100 Tests

Catalog No. BDB565120 Supplier BD Biosciences Supplier No. 565120

Description

Mouse Monoclonal Antibody

The UCHT1 monoclonal antibody specifically binds to the human CD3 ε -chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex. CD3 ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody is mitogenic for CD3 ε -positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody. CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3 ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3 ε .

This antibody was conjugated to BD Horizon APC-R700, which has been developed exclusively by BD Biosciences as a better alternative to Alexa Fluor™ 700. APC-R700 excites and emits at similar wavelengths to Alexa Fluor™ 700 yet exhibits significantly improved brightness. This dye can be excited by the red laser and detected with the same filter set as Alexa Fluor™ (eg, 730/45-nm filter).

Specifications

• Antigen: CD3
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: UCHT1
• Conjugate: APC-R700
• Description: CD3e; CD3E; T3E; TCRE; T-cell surface antigen T3/Leu-4 epsilon
• Formulation: Aqueous buffered solution containing BSA, protein stabilizer, glycerol, and ≤0.09% sodium azide.
• Host Species: Mouse
• Immunogen: Human infant thymocytes and peripheral blood lymphocytes from a Sezary Syndrome donor
• Purification Method: Affinity Purified
• Quantity: 25 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ

For Research Use Only.