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CD62L Mouse anti-Human, BB515, Clone: DREG-56, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 564742

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Catalog No. BDB564742


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Description

Description

The DREG-56 monoclonal antibody specifically binds to CD62L. CD62L is a 76-95 kDa glycoprotein that is also referred to as L-selectin or LECAM-1. CD62L is expressed on neutrophils, monocytes, T- and B-lymphocyte subsets and NK cells. The DREG-56 antibody recognizes the same antigen as LAM-1, and specifically inhibits >90% of binding of human lymphocytes to high endothelial venules (HEV) in frozen sections of peripheral, but not mucosal lymphoid tissue. It thus defines L-selectin as a human lymphocyte homing receptor for peripheral lymph node HEV.

The antibody was conjugated to BD Horizon™ BB515 which was developed exclusively by BD Biosciences. With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (eg, 530/30-nm filter). This dye provides a much brighter alternative to FITC with less spillover into the PE detector.

Specifications

Specifications

CD62L
DREG-56
SELL; L-selectin; LSEL; LAM-1; LECAM-1; LEU8; LNHR; MEL-14; PLNHR; TQ-1
Affinity Purified
Supernatant from PMA-activated Human Peripheral Blood Leukocytes
Affinity Chromatography
RUO
Primary
Human
Flow Cytometry
BB515
Aqueous buffered solution containing ≤0.09% sodium azide.
Murine
IgG1, κ
100 Tests
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
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