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CD16 Mouse anti-Human, Brilliant Violet 650, Clone: B73.1, BD Optibuild™

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Description

Mouse Monoclonal Antibody

The B73.1 monoclonal antibody specifically binds to CD16, the 50-70 kDa low affinity receptor for IgG, IgG Fc receptor III (Fc γRIII) . CD16 is expressed on NK cells, neutrophils, and on a subset of T cells from certain individuals. The B73.1 antibody binds to CD16-positive neutrophils with lower intensity when compared with some other CD16-specific antibodies. A variable number of CD16-positive lymphocytes coexpress either the CD57 antigen or low-density CD8 antigen or both. The B73.1 antibody can block Fc receptor functions mediated by CD16.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD16
Applications Flow Cytometry
Classification Monoclonal
Clone B73.1
Concentration 0.2mg/mL
Conjugate Brilliant Violet 650
Description IgG Fc receptor III; IGFR3; FCG3; FCGR3; FCGRIII; FcγRIII
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species Mouse
Immunogen Human NK Cells
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Human
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG1 κ
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only

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