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CD64 a and b Alloantigens Mouse anti-Mouse, Brilliant Violet 650, Clone: X54-5/7.1, BD Optibuild™

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 740622

Catalog No. BDB740622

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The X54-5/7.1 monoclonal antibody specifically recognizes FcγRI (CD64) encoded by the more common Fcgr1a and Fcgr1b alleles. The alloantigens generated by the Fcgr1a and Fcgr1b alleles, have been confirmed positive in mouse strains BALB/c and C57BL/6 and reported positive in strains 129, A, AKR, ALR, BUB, C3H, C57BL/10, C57BLKS, C57BR, C58, CBA, CE, DBA/2, HRS, MRL, NON, NZB, NZO, NZW, PL, SJL, ST, SWR. The a and b alloantigens have been reported negative in mouse strains ABH, NOD. CD64, a key receptor in the development of immune responses, has a dual role as a low affinity receptor for IgG3 and high affinity receptor for IgG2a linking innate and adaptive immunities. CD64 mediates endocytosis, phagocytosis, antibody-dependent cellular toxicity, cytokine release and superoxide generation. CD64 is expressed largely on macrophages and dendritic cells. For more information regarding clone X54-5/7.1 and the alloantigens it recognizes, please refer to the reference by Tan et al.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.



CD64 a and b Alloantigens
Brilliant Violet 650
Aqueous buffered solution containing ≤0.09% sodium azide.
IgG1, κ
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Flow Cytometry
Fcgr1; FcRI; Fcg1; Fc-gamma RI; Fc receptor IgG high affinity I; IGGHAFC
Affinity Purified
Mouse CD64 a Alloantigen
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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