MilliporeSigma™ Mouse anti-PERK, Clone: 4F6.1,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABN842

Catalog No. MABN842MI

Description

Specifically detects PERK clone: 4F6.1 in Human samples, and it is validated for Immunohistochemistry (Paraffin), Western Blotting

Eukaryotic translation initiation factor 2-alpha kinase 3 (EC 2.7.11.1; UniProt Q9NZJ5; also known as Eukaryotic translation initiation factor 2 alpha kinase 3, HsPEK, Pancreatic EIF2-alpha kinase, PRKR-like endoplasmic reticulum kinase) is encoded by the EIF2AK3 (also known as PEK, PERK, WRS) gene (Gene ID 9451) in human. The accumulation of unfolded or misfolded proteins in the endoplasmic reticulum (ER) induces the activation of three main unfold protein response (UPR) pathways, the inositol requiring enzyme-1 (IRE1) pathway, the activating transcription factor 6 (ATF6) pathway, and the protein kinase-like ER kinase (PERK) pathway. The PERK pathway involves PERK auto-phosphorylation/activation, resulting in PERK phosphorylation of eIF2α, which in turn leads to increased ATF4 transcription and ATF4-mediated CAAT/enhancer binding protein (C/EBP) homologous protein (CHOP) upregulation.

Specifications

• Antigen: PERK
• Classification: Monoclonal
• Formulation: Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Symbols: EIF2AK3; PEK; PERK
• Immunogen: GST-tagged recombinant protein corresponding to the Internal (C-terminal half) of human PERK.
• Quantity: 100 μg
• Research Discipline: Neuroscience
• Gene ID (Entrez): NP_004827
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
• Isotype: IgG2b κ

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Clone: 4F6.1
• Gene Accession No.: Q9NZJ5
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Form: Purified

For Research Use Only