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Invitrogen™ Mouse anti-Rat IgG2a Secondary Antibody, eFluor™ 615, eBioscience™

Mouse Monoclonal Secondary Antibody

$139.00

Specifications

Antigen Rat IgG2a
Clone r2a-21B2
Concentration 0.2 mg/mL
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Applications Immunohistochemistry (Paraffin), Immunocytochemistry, Immunohistochemistry (Frozen)
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Products 1
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-113-0717
Encompass_Preferred
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Invitrogen™
42481780
25 μg
Each of 1 for $139.00
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Description

Description

Description: The monoclonal antibody r2a-21B2 recognizes Rat IgG2a antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG2a or any mouse isotype antibodies. Applications Reported: This R2a-21B2 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry. Applications Tested: This R2a-21B2 antibody has been tested by immunocytochemistry and immunohistochemistry using a rat primary antibody at 10 μg/mL. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor™ 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor™ 594 filter is acceptable. Excitation: 595 nm; Emission: 615 nm. Filtration: 0.2 μm post-manufacturing filtered.

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Specifications

Specifications

Rat IgG2a
0.2 mg/mL
Immunohistochemistry (Paraffin), Immunocytochemistry, Immunohistochemistry (Frozen)
eFluor 615
Mouse
RUO
PBS with 0.09% sodium azide; pH 7.2
Secondary Antibody
r2a-21B2
4°C, store in dark, DO NOT FREEZE!
Monoclonal
Liquid
IgG1 κ
Rat
Secondary
Affinity chromatography
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