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Mouse brain tissue lysate, Non-denatured; Abnova
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Quantity:
200 μg
Description
- Tissue: Brain
- Host: Mouse
- Lysis buffer: Modified RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
- Storage buffer: In modified RIPA lysis buffer
Western Blotting, Immunoprecipitation
Specifications
Specifications
For Use With (Application) | Immunoprecipitation, Western Blot |
Tissue | Brain |
Description | Whole tissue lysate (non-denatured) |
Lysis Buffer | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. ;Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
Preparation Method | Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 5 mg/ml. |
Quality Control Testing | 12.5% SDS-PAGE Stained with Coomassie Blue. |
Quantity | 200 μg |
Storage Buffer | In modified RIPA Lysis Buffer. |
Host Species | Mouse |
Content And Storage | Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
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