Invitrogen™ Mouse CD200 His-tag Recombinant Protein

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (1786 μL) be added to the vial to prepare a stock solution of 0.56 mg/mL. Concentration is measured by UV.

CD200 is a type-1 membrane glycoprotein that contains two immunoglobulin domains, and belongs to the immunoglobulin superfamily. CD200 is a broadly distributed cell surface glycoprotein that interacts with a receptor on myeloid cells (CD200R) and is implicated in locally preventing macrophage activation. Distant, but recognizable, homologues of CD200 have been identified in many herpesviruses and poxviruses. Studies of the related genes in mouse and rat suggest that CD200 may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. Multiple alternatively spliced transcript variants that encode different isoforms of CD200 have been found. Diseases associated with CD200 dysfunction include Mantle Cell Lymphoma and Primary Hypertrophic Osteoarthropathy.

Specifications

• Accession Number: O54901
• For Use With (Application): ELISA
• Format: Lyophilized
• Formulation: PBS with 0.01% mannitol, 0.01% Tween 80, 5-8% trehalose and no preservative; pH 7.4
• Gene ID (Entrez): 17470
• Molecular Weight (g/mol): 24 kDa
• Name: Mouse CD200 His-tag
• Purification Method: SDS-PAGE
• Quantity: 100 μg
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: antigen identified by monoclonal antibody MRC OX-2; CD200; Cd200 antigen; Cd200 molecule; cell surface protein (thymocyte antigen identified by monoclonal antibody MRC-O x 2); Cspmo2; hypothetical protein LOC534910; MO x 1; MO x 2; MRC; MRC OX-2 antigen; MRCO x 2; My033; O x 2; OX-2; OX-2 membrane glycoprotein
• Common Name: CD200
• Gene Symbol: Cd200
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse CD200 (at 1 μg/ml (100 μL/well) can bind mouse CD200R1/Fc with a linear range of 1.5-200 ng/mL)
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Mouse CD200, amino acids Met1-Gly232 (Accession # O54901.1) with a C-terminal His-tag
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse CD200 (at 1 μg/ml (100 μL/well) can bind mouse CD200R1/Fc with a linear range of 1.5-200 ng/mL)
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >95% as determined by SDS-PAGE.