Invitrogen™ Mouse EphA3 His-tag Recombinant Protein

Catalog No.	Quantity
PIRP88232	200 μg

Catalog No. PIRP88232 Supplier Invitrogen™ Supplier No. RP88232

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (800 μL) be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV.

EPHA3 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediated developmental events, particularly in the nervous system. EPHA3 encodes a protein that binds ephrin-A ligands and receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EPHA3 binds ephrin-A ligands.

Specifications

• Accession Number: P29319
• For Use With (Application): Control, ELISA Standard, Binding Assay
• Format: Lyophilized
• Formulation: PBS with 0.01% mannitol, 5-8% trehalose, 0.01% Tween 80 and no preservative; pH 7.4
• Gene ID (Entrez): 13837
• Molecular Weight (g/mol): 60.2 kDa
• Name: Mouse EphA3 His-tag
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 200 μg
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: AW492086; Cek4; EK4; End3; EPH receptor A3; EPHA3; EPH-like kinase 4; Eph-like tyrosine kinase 1; Ephrin type-A receptor 3; ETK; ETK1; H TYRO4; Hek; Hek4; Human embryo kinase; human embryo kinase 1; I79_002835; Mek4; rEK4; testicular tissue protein Li 64; TYRO4; TYRO4 protein tyrosine kinase; tyrosine-protein kinase receptor ETK1; tyrosine-protein kinase receptor REK4; tyrosine-protein kinase TYRO4
• Common Name: EphA3
• Gene Symbol: EPHA3
• Biological Activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse EPHA3-His (at 10 μg/mL (100 μL/well) can bind mouse EFNA5-Fc . The EC50 of mouse EFNA5-Fc is 4.9-11.4 ng/mL)
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Mouse EPHA3, amino acids Met1-His541 (Accession # NP_034270.1) with a C-terminal His-tag
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse EPHA3-His at 10 μg/mL (100 μL/well) can bind mouse EFNA5-Fc . The EC50 of mouse EFNA5-Fc is 4.9-11.4 ng/mL.
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >95% as determined by SDS-PAGE.