Invitrogen™ Mouse Ferritin ELISA Kit

ELISA

Supplier:  Invitrogen™ EEL095

Catalog No. EEL095

Description

Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. Ferritin is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in the light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. The ferritin gene has multiple pseudogenes, and ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis. Ferritin is a holoenzyme shell with a molecular weight of about 450 kDa, and consists of 24 subunits of two types, H(heavy) and L(light), and capable of storing up to 4,500 atoms of ferric iron.

Specifications

• Accession Number: P09528, P29391
• Assay Sensitivity: 0.94 ng/mL
• Product Type: ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: FTH1, Ftl1
• Intraassay CV: <10
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Test Time: 1 hrs 20 mins

• Assay Range: 1.56 to 100 ng/mL
• Conjugate: HRP
• Sample Type: Plasma, Serum
• Gene ID (Entrez): 14319, 14325
• Interassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Quantity: 96 Tests
• Sample Volume: Plasma 100 μL, Serum 100 μL
• Target: Ferritin
• Total Assay Time: 3 hrs 30 mins