Gibco™ Mouse FGF-basic (FGF-2/bFGF) Recombinant Protein, PeproTech®

Catalog No.	Quantity
45033100UG	100 μg
450-33-10UG	10 μg
45-033-01M	2 x 500 μg
45033250UG	250 μg
45033500UG	500 μg
450-33-50UG	50 μg

Catalog No. 45033100UG Supplier Gibco™ Supplier No. 45033100UG

Description

Recombinant Protein

450-33-1MG will be provided as 2 x 500 μg (450-33-500UG). Recombinant Murine FGF-basic is a 16.3 kDa protein consisting of 145 amino acid residues. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor (FGF) family, and interacts with high-affinity transmembrane receptors to influence cell proliferation and tissue neovascularization. FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is predominantly cytosolic and acts through cell surface receptors, whereas the 22, 22.5, 24 and 34 kDa isoforms are nuclear and may signal independent of transmembrane receptor pathways. In humans, the gene is located on the q arm of chromosome 4. FGF2 has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for FGF2 contains multiple polyadenylation sites, and is alternatively translated from non-AUG and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.

Specifications

• Accession Number: P15655
• For Use With (Application): Control, Bioactivity
• Formulation: Protein with no preservative
• Gene ID (Entrez): 14173
• Molecular Weight (g/mol): 16.3 kDa
• Name: Mouse FGF-basic (FGF-2/bFGF)
• Purification Method: Purified
• Quantity: 100 μg
• Source: E. coli
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/μg
• Gene Alias: Basic fibroblast growth factor; basic fibroblast growth factor bFGF; bFGF; FGF; fgf basic; Fgf2; Fgf-2; Fgfb; FGF-b; Fibroblast growth factor; fibroblast growth factor 2; fibroblast growth factor 2 (basic); Fibroblast growth factor basic; HBGF-2; Heparin-binding growth factor 2; H-FGF-b-147; H-FGF-b-154; M-FGF-b; Prostatic growth factor; prostatropin
• Common Name: FGF2
• Gene Symbol: FGF2
• Biological Activity: Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10^6 units/mg
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: PALPEDGGAA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHV KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTEE CFFFERLESN NYNTYRSRKY SSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
• Content And Storage: -20°C
• Activity: Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10^6 units/mg.
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 95% by SDS-PAGE gel and HPLC analyses.