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Invitrogen™ Mouse Hematopoietic Lineage Antibody Cocktail, eFluor™ 450, eBioscience™
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Catalog No. 501129718
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50-112-9718 50 Tests
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Catalog No. 50-112-9718 Supplier Invitrogen™ Supplier No. 88777272
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Description

Antibody Cocktail

Description: The Mouse Hematopoietic Lineage eFluor™ 450 Cocktails contains eFluor™ 450-conjugated antibodies that can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on the expression of cell surface antigens. This cocktail contains the following antibodies: Anti-Mouse CD3 (17A2) eFluor™ 450 Anti-Mouse CD45R (B220) (RA3-6B2) eFluor™ 450 Anti-Mouse CD11b (M1/70) eFluor™ 450 Anti-Mouse TER-119 (TER-119) eFluor™ 450 Anti-Mouse Ly-G6 (Gr-1) (RB6-8C5) eFluor™ 450 Applications Reported: The Mouse Hematopoietic Lineage eFluor™ 450 Cocktail has been reported for use in flow cytometric analysis. Applications Tested: The Mouse Hematopoietic Lineage eFluor™ 450 Cocktail has been pre-titrated and tested by flow cytometric analysis of C57Bl/6 bone marrow cells. This can be used at 20 μL per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Hematopoietic Lineage antibodies can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on their expression of cell surface antigens.
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Specifications

Antigen Mouse Hematopoietic Lineage
Applications Flow Cytometry
Classification Cocktail
Concentration 20 μL/Test
Conjugate eFluor 450
Formulation PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias 17A2; Hematopoietic Lineage Cocktail; M1/70; RA3-6B2; RB6-8C5; TER-119
Host Species Rat
Purification Method Affinity chromatography
Quantity 50 Tests
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
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