Invitrogen™ Mouse IFN-gamma (ELISA Standard) Recombinant Protein

Catalog No.	Quantity
50-112-8733	4 pack

Catalog No. 50-112-8733 Supplier Invitrogen™ Supplier No. 39831165

Description

Recombinant Protein

Description: Mouse IFN-gamma is a ~20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN-gamma, in synergy with other cytokines such as TNF-alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma. Glycosylation of IFN-gamma does not affect its biological activity. Applications Reported: Recombinant mouse IFN gamma is biologically active. The intended use of the Recombinant Mouse IFN gamma Single-Use ELISA RSG Standard is as a standard for mouse IFN gamma Ready-SET-Go! ELISA and mouse IFN gamma matched antibody pair ELISA. Typically the standard is run in a series of 7 doubling dilutions from the 'top' standard described below. Instructions for use (XMG1.2/R4-6A2 assays): For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 20 μL of standard solution to 10 mL of assay diluent to prepare the top standard solution (2000 pg/mL).For matched antibody pair ELISA (ABTS or Super AquaBlue substrate). Dilution: Add 20 μL of standard solution to 10 mL of assay diluent to prepare the top standard solution (2000 pg/mL). Instructions for use (AN-18/R4-6A2 assays): For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 20 μL of standard solution to 10 mL of assay diluent to prepare 2000 pg/mL solution. Mix thoroughly.

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

Specifications

• Accession Number: P01580
• For Use With (Application): Neutralization, ELISA Standard
• Formulation: Protein with 0.09% sodium azide
• Gene ID (Entrez): 15978
• Molecular Weight (g/mol): 20 kDa
• Name: Mouse IFN-gamma (ELISA Standard)
• Purification Method: Purified
• Quantity: 4 pack
• Source: E. Coli
• Storage Requirements: 2-8°C
• Regulatory Status: RUO
• Gene Alias: BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN γ; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFNγ; Immune interferon; Interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon γ; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferonγ; M-IFN-g; R-IFN-g
• Common Name: IFN gamma
• Gene Symbol: IFNG
• Product Type: Protein
• Conjugate: Unconjugated
• Species: E. coli
• Recombinant: Recombinant
• Sequence: Mouse IFN-gamma, amino acids His23-Cys155 (Accession #NM_008337)
• Content And Storage: -80°C
• Expression System: E. coli
• Form: Lyophilized
• Protein Subtype: Recombinant
• Purity or Quality Grade: >98% by SDS-PAGE