Ig Goat anti-Mouse, BB515, Polyclonal, BD

Goat Polyclonal Antibody

Supplier:  BD Biosciences 565060

Catalog No. BDB565060

Description

BD Horizon™ BB515 Goat Anti-Mouse Ig is intended to be a second-step reagent for immunofluorescent staining of cells pre-stained with Mouse Ig primary antibodies. It is reactive with the heavy and light chains of mouse primary antibodies having the IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA isotypes. Minimal background staining of human and rat cells occurs in the absence of a primary mouse antibody. In addition, the BD Horizon BB515 Goat Anti-Mouse Ig stains mouse B lymphocytes with little non-specific staining of other cells. Therefore, it is useful as a primary immunofluorescent reagent for staining mouse B cells and antibody-producing cells and as a secondary reagent for staining human and rat cells pre-stained with mouse Ig primary antibodies.

The antibody was conjugated to BD Horizon BB515 which was developed exclusively by BD Biosciences. With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (e.g. 530/30-nm filter). This dye provides a much brighter alternative to FITC with less spillover into the PE detector.

Specifications

• Antigen: Ig
• Classification: Polyclonal
• Conjugate: Brilliant Blue 515 (BB515)
• Host Species: Goat
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: Ig