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Invitrogen™ Mouse IgD Monoclonal Antibody (11-26c (11-26)), Brilliant Ultra Violet™ 496, eBioscience™

Catalog No. 364599380
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36-459-9380 25 μg
36-459-9382 100 μg
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Catalog No. 36-459-9380 Supplier Invitrogen™ Supplier No. 364599380
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Rat Monoclonal Antibody

The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells.Applications TestedThis 11-26c (11-26) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 g per test. A test is defined as the amount (g) of antibody that will stain a cell sample in a final volume of 100 L. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Blocking BuffersWhen using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.Light sensitivityThis tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 L of cell sample + 100 L of IC Fixation Buffer) or...

This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Mouse IgD
Applications Flow Cytometry
Classification Monoclonal
Clone 11-26c (11-26)
Concentration 0.2 mg/mL
Conjugate Brilliant Ultraviolet 496
Formulation PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Ighd
Gene Accession No. P01881
Gene Alias IgD; Igh-5; IGHD; immunoglobulin heavy constant delta
Gene Symbols Ighd
Host Species Rat
Purification Method Affinity chromatography
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 380797
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG2a κ
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