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Invitrogen™ Mouse IgD Monoclonal Antibody (11-26c (11-26)), Brilliant Ultra Violet™ 496, eBioscience™
Description
The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells.Applications TestedThis 11-26c (11-26) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 g per test. A test is defined as the amount (g) of antibody that will stain a cell sample in a final volume of 100 L. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Blocking BuffersWhen using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.Light sensitivityThis tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 L of cell sample + 100 L of IC Fixation Buffer) or...
Specifications
Specifications
| Antigen | Mouse IgD |
| Applications | Flow Cytometry |
| Classification | Monoclonal |
| Clone | 11-26c (11-26) |
| Concentration | 0.2 mg/mL |
| Conjugate | Brilliant Ultraviolet 496 |
| Formulation | PBS with BSA and 0.09% sodium azide; pH 7.2 |
| Gene | Ighd |
| Gene Accession No. | P01881 |
| Gene Alias | IgD; Igh-5; IGHD; immunoglobulin heavy constant delta |
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