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Invitrogen™ Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine5, eBioscience™

Catalog No. 501129378
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50-112-9378 50 μg
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Catalog No. 50-112-9378 Supplier Invitrogen™ Supplier No. 15471481
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Description

Mouse Monoclonal Isotype Control

The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control. Applications Reported: PE-Cy5 Mouse IgG1 K Isotype Control has been reported for use in flow cytometric analysis. Applications Tested: Thhis Mouse IgG1 K Isotype Control can be used at the same concentration as the experimental antibody. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Mouse IgG1 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG1 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG1 is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Mouse IgG1 kappa
Applications Control, Flow Cytometry
Classification Monoclonal
Clone P3.6.2.8.1
Concentration 0.2 mg/mL
Conjugate PE-Cyanine5
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene Alias IgG1; Igh-4; Ighg1; Immunoglobulin G; Immunoglobulin G1; immunoglobulin heavy constant gamma 1 (G1m marker); ImmunoglobulinG; ImmunoglobulinG1; VH7183
Host Species Mouse
Purification Method Affinity chromatography
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Isotype Control (Primary)
Target Species Not Applicable
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Isotype Control
Form Liquid
Isotype IgG1 κ
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