Invitrogen™ Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 780, eBioscience™

Mouse Monoclonal Isotype Control

Supplier:  Invitrogen™ 78471482

Catalog No. 78-471-482

Description

Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control. Applications Reported: This P3.6.2.8.1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This P3.6.2.8.1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse splenocytes. Use at the same concentration as the experimental antibody. Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm. We recommend using a 780/60 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres (Product # 01-2222-42) as compared to single-color stained cells. In such circumstances, we would recommend setting compensation with cells. We have also observed this in some experiments using AbC Total Antibody Compensation beads (Product # A10497). Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained sam...

Mouse IgG1 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG1 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG1 is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.

Specifications

• Antigen: Mouse IgG1 kappa
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Isotype Control (Primary)
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Control, Flow Cytometry
• Clone: P3.6.2.8.1
• Conjugate: Super Bright 780
• Gene Alias: IgG1; Igh-4; Ighg1; Immunoglobulin G; Immunoglobulin G1; immunoglobulin heavy constant gamma 1 (G1m marker); ImmunoglobulinG; ImmunoglobulinG1; VH7183
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Not Applicable
• Product Type: Isotype Control
• Isotype: IgG1 κ