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Invitrogen™ Mouse IgG2a kappa Isotype Control (eBM2a), Alexa Fluor™ 561, eBioscience™
Mouse Monoclonal Isotype Control
Supplier: Invitrogen™ 505472481
Description
Description: This is a monoclonal mouse IgG2a kappa antibody. It is used as an isotype control for mouse IgG2a antibodies. Applications Reported: This eBM2a antibody has been reported for use in flow cytometric analysis. Our internal testing shows that Alexa Fluor 561 non-specifically stains B cells in Swiss Webster and SJL mice. Non-specific staining has not been observed in BALB/c or C57BL/6 mice. Other strains have not been tested. See the Antibody Testing Data for an example of this strain-dependent difference. Applications Tested: This eBM2a antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells and should be used at the same concentration as the experimental antibody. Alexa Fluor 561 emits at 575 nm and is intended for use on spectral cytometers where it may be multiplexed with both PE and PE-eFluor 610. Excitation: 558 nm; Emission: 575 nm; Laser: Yellow-Green Laser.
Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2a is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2a is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
Specifications
| Mouse IgG2a kappa | |
| Monoclonal | |
| 0.2 mg/mL | |
| PBS with 0.09% sodium azide; pH 7.2 | |
| Mouse | |
| 50 μg | |
| Isotype Control (Primary) | |
| 4°C, store in dark, DO NOT FREEZE! | |
| Liquid |
| Control, Flow Cytometry | |
| eBM2a | |
| Alexa Fluor 561 | |
| IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2 | |
| Affinity chromatography | |
| RUO | |
| Not Applicable | |
| Isotype Control | |
| IgG2a κ |
Safety and Handling
WARNING: Cancer - www.P65Warnings.ca.gov
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