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R&D Systems™ Mouse IL-1 beta/IL-1F2 Quantikine HS ELISA Kit

Catalog No. MHSLB00
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Description

From the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

  • A 4.0 hour solid phase ELISA designed to measure mouse IL-1 beta levels in serum and plasma.
  • It contains E. coli-expressed recombinant mouse IL-1 beta and antibodies raised against the recombinant protein.
  • Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine™ HS kit standards.
  • These results indicate that the Quantikine™ HS Immunoassay kit can be used to determine relative mass values for natural mouse IL-1 beta.
  • Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
  • Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians.

Specifications

Assay Sensitivity 0.312 pg/mL
Cross Reactivity < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Product Type ELISA
Specificity Natural and recombinant mouse IL-1 beta
Gene ID (Entrez) 3553
Host Species Mouse
Quantity 1 Kit
Sample Volume Serum (50 μL), EDTA Plasma (50 μL), Heparin Plasma(50 μL)
Storage Requirements Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Synonym catabolin, IL-1, IL1 beta, IL-1 beta, IL1B, IL-1B, IL1-BETA, IL-1F2, IL1F2IL-1 beta, interleukin 1, beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta
Target IL-1 beta/IL-1F2
Total Assay Time 4 hours
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