R&D Systems™ Mouse IL-1 beta/IL-1F2 Quantikine HS ELISA Kit

Description

From the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

• A 4.0 hour solid phase ELISA designed to measure mouse IL-1 beta levels in serum and plasma.
• It contains E. coli-expressed recombinant mouse IL-1 beta and antibodies raised against the recombinant protein.
• Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine^™ HS kit standards.
• These results indicate that the Quantikine^™ HS Immunoassay kit can be used to determine relative mass values for natural mouse IL-1 beta.
• Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians.

Specifications

• Assay Sensitivity: 0.312 pg/mL
• Cross Reactivity: < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
• Product Type: ELISA
• Specificity: Natural and recombinant mouse IL-1 beta
• Gene ID (Entrez): 3553
• Host Species: Mouse
• Quantity: 1 Kit
• Sample Volume: Serum (50 μL), EDTA Plasma (50 μL), Heparin Plasma(50 μL)
• Storage Requirements: Store the unopened product at 2 - 8 °C. Do not use past expiration date.
• Synonym: catabolin, IL-1, IL1 beta, IL-1 beta, IL1B, IL-1B, IL1-BETA, IL-1F2, IL1F2IL-1 beta, interleukin 1, beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta
• Target: IL-1 beta/IL-1F2
• Total Assay Time: 4 hours