Invitrogen™ eBioscience™ Mouse IL-2 ELISA Ready-SET-Go!™ Kit 

Specifically engineered for accurate and precise measurement of mouse IL-2 protein levels from samples including serum, and cell culture supernatants.

Manufacturer:  Invitrogen™ 88702486

Catalog No. 50-112-5174

Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant protein for generating standard curve and calibrating samples
10X Coating Buffer . Buffer for plating the Capture Antibody
5X ELISA/ELISPOT Diluent . Buffer for blocking and diluting the Detection Antibody and Enzyme
Enzyme. Pre-titrated Avidin-HRP
Substrate. 1X TMB Solution

Description

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

Specifications

• Accession Number: P04351
• Conjugate: Biotin
• Gene ID (Entrez): 16183
• For Use With (Equipment): Colorimetric Microplate Reader
• Product Type: ELISA Kit
• Regulatory Status: RUO
• Sample Type: Serum,Supernatant
• Species: Mouse
• Test Time: 1 hr 45 min
• Target: Mouse
• Synonym: Interleukin-2

• Assay Range: 2-200 pg/mL
• For Use With (Application): ELISA
• Includes: 96-well plates
• Kit Contents: 10X Coating Buffer: Buffer for plating the Capture Antibody
• Quantity: 10 x 96 tests
• Sensitivity: 2 pg/mL
• Sample Volume: Serum,100 μL|Supernatant,100 μL
• Storage Requirements: 2-8°C
• Total Assay Time: 24 hr 30 min
• Gene Symbols: Il2
• Label or Dye: HRP

For Research Use Only