Invitrogen™ Mouse IL-27 p28 Recombinant Protein

Catalog No.	Quantity
PIRP8611	10 μg

Catalog No. PIRP8611 Supplier Invitrogen™ Supplier No. RP8611

Description

Recombinant Protein

IL-27 (mouse) Recombinant Protein, RP-8611, from Invitrogen.

IL-27, a member of the IL-12 family, is a heterodimeric protein consisting of the p40-related protein Epstein-Barr virus-induced gene 3 (EBI3) non-covalently linked to an IL-12p35-related protein, p28 (also known as IL-30). IL-27 is produced by activated APCs and mature dendritic cells. IL-27 exerts its activities on NK cells and naive CD4+ T cells; mRNA expression analysis of IL-27 receptor components (WSX-1/TCCR and gp130) suggests that IL-27 may also target other cells, including mast cells and monocytes. Binding of IL-27 to WSX-1/gp130 activates JAK1, STAT1, and STAT3 and STAT1/3 phosphorylation. WSX-1/TCCR-deficient mice develop impaired Th1 responses and are more susceptible to infection with L. monocytogenes suggesting that Th1 responses require IL-27. Although activation of WSX-1 is required for the initiation of Th1 responses, it is not necessary for maintaining Th1 responses. IL-27 alone is not able to induce the differentiation of CD4+ T cells into IFN-γ-producing cells, suggesting a role for IL-27 as an initial activator of Th1 responses. An important effect of IL-27 in initiating Th1 responses is the induction of the Th1-specific transcription factor T-bet as well as the suppression of the Th2-specific transcription factor GATA-3. T-bet plays a critical role in Th1 differentiation by its ability to maintain IL-12Rβ2 expression following CD4+ T cell activation. Recent studies indicate that IL-27 has a potent antitumor activity. In vitro, IL-27 has been found to act directly on naive CD8 cells, generating CTL with enhanced granzyme B expression. In vivo, IL-27 has been reported to augment CTL activity, inhibit tumor growth, and induce complete regression of primary and metastatic neuroblastoma tumors.

Specifications

• Accession Number: Q8K3I6
• Concentration: 0.1 mg/mL
• For Use With (Application): Control
• Formulation: 10 mM sodium bicarbonate with no preservative; pH 8.5
• Gene ID (Entrez): 246779
• Molecular Weight (g/mol): 23.7 kDa
• Name: Mouse IL-27 p28
• pH Range: 8.5
• Quantity: 10 μg
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: ≤1 EU/ μg
• Gene Alias: IL27; IL-27; IL-27 p28 subunit; IL-27 subunit alpha; Il27a; IL-27 A; IL-27-A; IL27-A; IL27p28; IL-27p28; IL30; IL-30; ILN; Interleukin; interleukin 27; interleukin 30; Interleukin-27; interleukin-27 subunit alpha; Interleukin-30; p28
• Common Name: IL-27 p28
• Gene Symbol: Il27
• Conjugate: Unconjugated
• Species: E. coli
• Recombinant: Recombinant
• Sequence: MFPTDPLSLQ ELRREFTVSL YLARKLLSEV QGYVHSFAES RLPGVNLDLL PLGYHLPNVS LTFQAWHHLS DSERLCFLAT TLRPFPAMLG GLGTQGTWTS SEREQLWAMR LDLRDLHRHL RFQVLAAGFK CSKEEEDKEE EEEEEEEEKK LPLGALGGPN QVSSQVSWPQ LLYTYQLLHS LELVLSRAVR DLLLLSLPRR PGSAWDS
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Lyophilized
• Protein Subtype: Recombinant
• Purity or Quality Grade: ≥95% by Reducing and Non-Reducing SDS PAGE