R&D Systems™ Recombinant Mouse IL-6/IL-6R alpha Protein Chimera

Catalog No.	Quantity
90-38S-R025	25 μg

Catalog No. 90-38S-R025 Supplier R&D Systems™ Supplier No. 9038SR025

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

The Recombinant Mouse IL-6/IL-6R alpha Protein Chimera is derived from NS0. The Recombinant Mouse IL-6/IL-6R alpha Protein Chimera has been validated for the following applications: Bioactivity.

Specifications

• For Use With (Application): Bioactivity
• Molecular Weight (g/mol): Observed M.W.: 70-87kDa, reducing conditions, Theoretical M.W.: 60kDa
• Name: IL-6/IL-6R alpha Complex
• Quantity: 25 μg
• Source: Mouse myeloma cell line,NS0-derived mouse IL-6/IL-6R alpha Complex protein, (N-terminus) Mouse IL-6R alpha (Leu20-Glu357) Accession &Num; P22272 GGGSGGGSGGGS Mouse IL-6 (Phe25-Thr211) Accession &Num; P08505 (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Common Name: IL-6/IL-6 R alpha Complex
• Gene Symbol: Il6
• Biological Activity: Measured by its ability to inhibit M1 mouse myeloid leukemia cell proliferation. The ED50 for this effect is typically 2-12ng/mL.
• Conjugate: Unconjugated
• Species: Mouse
• Recombinant: Recombinant
• Expression System: Mouse myeloma cell line, NS0-derived Mouse IL-6 R alpha (Leu20-Glu357) Accession # P22272, N-terminus; GGGSGGGSGGGS; Mouse IL-6 (Phe25-Thr211) Accession # P08505, C-terminus
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining