Invitrogen™ Mouse MCP-3 Instant ELISA™ Kit

Catalog No.	Quantity
50-182-74	128 Tests

Catalog No. 50-182-74 Supplier Invitrogen™ Supplier No. BMS6006INST

Description

ELISA

The Mouse Moncyte Chemoattractant Protein 3 (Ms MCP-3) ELISA quantitates Ms MCP-3 in mouse serum, plasma, cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Ms MCP-3. Principle of the method The Mouse MCP-3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.

Specifications

• Accession Number: Q03366
• Assay Range: 15.6 to 1,000 pg/mL
• Assay Sensitivity: 2.6 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Mouse
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 20306
• Gene Symbol: MCP-3,Scya7,fic,marc,mcp3
• Interassay CV: 9.5%
• Intraassay CV: 7.7
• Kit Contents: Pre-coated 96 well Microplate, Biotinylated Detection Antibody, SAV-HRP, Sample Diluent, lyophilized, Standard (8-well strips x 4), Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 128 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: -20°C
• Synonym: FIC, MARC, CCL7, Monocyte chemotactic protein 3
• Target: CCL7 (MCP3)
• Test Time: 15 mins
• Total Assay Time: 3 hrs 10 mins