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  5. Invitrogen™ Mouse SLAM/CD150 ELISA Kit 

Invitrogen™ Mouse SLAM/CD150 ELISA Kit

Catalog No. EM70RB
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EM70RB 96 Tests
EM70RBX5 5 x 96 Tests
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Catalog No. EM70RB Supplier Invitrogen™ Supplier No. EM70RB
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Description

ELISA

Mouse SLAM/CD150 quantitates mouse SLAM/CD150 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant mouse SLAM/CD150.

CD150 (SLAM, Signaling Lymphocyte Activation Molecule) belongs to the immunoglobulin gene superfamily, and is involved in T cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane bound form, the soluble form and the cytoplasmic isoforms of SLAM. It is suggested that signalling through homophilic SLAM-SLAM binding during B to B and B to T cell interactions enhances the expansion and differentiation of activated B cells. Reports suggest that the extracellular domain of CD150 is the receptor for the measles virus and acts as a co-activator on T cells and B cells. Moreover, CD150 is thought to interact with SH2D1A and with PTPN11 via its cytoplasmic domain. Mutations CD150 gene may be associated with X-linked lympho-proliferative disease (XLP).
TRUSTED_SUSTAINABILITY

Specifications

Accession Number 4933415F16; AA177906; CD150; CD150 antigen; CDw150; ESTM51; IPO-3; RGD1560634; signaling lymphocyte activation molecule; signaling lymphocytic activation molecule; signaling lymphocytic activation molecule family member 1; Slam; SLAM family member 1; SLAMF1
Assay Range 0.06 to 12 ng/mL
Assay Sensitivity 0.06 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 27218
Gene Symbol Slamf1
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target CD150
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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