Gibco™ Mouse Sonic Hedgehog C25II Recombinant Protein, PeproTech®

Catalog No.	Quantity
31-522-01M	4 x 250 μg
31522100UG	100 μg
31522250UG	250 μg
31-522-500	2 x 250 μg
315-22-25UG	25 μg
315-22-5UG	5 μg

Catalog No. 31-522-01M Supplier Gibco™ Supplier No. 3152201M

Description

Recombinant Protein

315-22-1MG will be provided as 4 x 250 μg (315-22-250UG). 315-22-500UG will be provided as 2 x 250 μg (315-22-250UG). Recombinant Murine Sonic Hedgehog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the naturally occurring, chemically modified Cys residue. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Sonic Hedgehog (SHH), which is expressed only during embryogenesis, is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the Sonic Hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.

Specifications

• Accession Number: Q62226
• For Use With (Application): Control, Bioactivity
• Formulation: Protein with no preservative
• Gene ID (Entrez): 20423
• Molecular Weight (g/mol): 20 kDa
• Name: Mouse Sonic Hedgehog C25II
• Purification Method: Purified
• Quantity: 4 x 250 μg
• Source: E. coli
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/μg
• Gene Alias: 9530036O11Rik; Dsh; fc83d08; hedgehog; hemimelic extra toes; Hhg1; HHG-1; HLP3; HPE3; Hx; Hxl3; M100081; MCOPCB5; morphogen; shh; Shh N-terminal processed signaling domains; Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; shh.L; shha; ShhN; ShhNC; ShhNp; short digits; SMMCI; sonic hedgehog; sonic hedgehog a; sonic hedgehog homolog; sonic hedgehog L homeolog; sonic hedgehog protein; Sonic hedgehog protein 19 kDa product; Sonic hedgehog protein A; Sonic hedgehog protein A N-product; Sonic hedgehog protein N-product; sonic hedgehog signaling molecule; sonic hedgehog signaling molecule a; sonic-you; syu; TPT; TPTPS; vhh1; vhh-1; wu:fc83d08; XELAEV_18030676mg; Xhh; xshh; X-SHH
• Common Name: SHH
• Gene Symbol: Shh
• Biological Activity: Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 0.5-1.0 ug/ml
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
• Content And Storage: -20°C
• Activity: Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 0.5-1.0 ug/ml.
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 90% by SDS-PAGE gel and HPLC analyses.