Deoxyribonuclease I from Bovine pancreas, MP Biomedicals™

Description

Deoxyribonuclease I from Bovine pancreas 50,000 Dornase u/mg solid, White powder

• Deoxyribonuclease from beef pancreas, DNase I, was first crystallized by Kunitz.
• It is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'.
• The average chain of limit digest is a tetranucleotide. DNase I acts upon single chain DNA, and upon double-stranded DNA and chromatin.
• In the latter case, although histones restrict susceptibility to nuclease action, over a period of time nearly all chromatin DNA is acted upon.
• According to Mirsky and Silverman, this could result from the looseness of histone attachment to DNA.
• They found that lysine-rich histones more effectively block DNase access to DNA than arginine-rich histones

Specifications

• Color: White
• pH: 7.8
• For Use With (Application): Used as a genetic marker for forensic identification
• Content And Storage: 0°C
• Source: Bovine pancreas
• Quantity: 250 mg
• Product Type: Deoxyribonuclease I
• Molecular Weight (g/mol): 31000
• Inhibitors: EDTA 7,8 and Sodium Dodecyl Sulfate
• Synonym: DNase I
• Form: Lyophilized Powder