Deoxyribonuclease I from Bovine pancreas, MP Biomedicals™

Description

Deoxyribonuclease I from Bovine pancreas 50,000 Dornase u/mg solid, White powder

• Deoxyribonuclease from beef pancreas, DNase I, was first crystallized by Kunitz.
• It is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'.
• The average chain of limit digest is a tetranucleotide. DNase I acts upon single chain DNA, and upon double-stranded DNA and chromatin.
• In the latter case, although histones restrict susceptibility to nuclease action, over a period of time nearly all chromatin DNA is acted upon.
• According to Mirsky and Silverman, this could result from the looseness of histone attachment to DNA.
• They found that lysine-rich histones more effectively block DNase access to DNA than arginine-rich histones

Specifications

• Color: White
• pH: 7.8 optimum (literature)
• CAS: 9003-98-9
• Content And Storage: Store at -0°C.
• MDL Number: MFCD00130918
• SMILES: *
• IUPAC Name: Deoxyribonuclease
• Molecular Weight (g/mol): 0.00
• Quantity: 0.5 mU
• Synonym: Deoxyribonucleate 5′-oligonucleotido-hydrolase
• Form: Powder