MP Biomedicals™ Peroxidase From Horseradish

Description

Useful for tracing neural connections, amplifying weak signals and increasing detectability of a target molecule such as a protein

A plant glycohemoprotein, Horseradish Peroxidase's (HRP's) enzymatic activity is due to the cyclic reduction and oxidation of the iron atom in the hematin group. The iron-containing hemin group is covalently bound to the glycoprotein apoenzyme. HRP readily combines with hydrogen peroxide and the resultant complex can oxidize a wide variety of chromogenic hydrogen donors. It is composed of seven isozymes, all containing photohemin IX as prosthetic group.

• Specific activity: ≥60 purpurogallin units/mg
• Extinction coefficient: EmM = 100 (403 nm)(Lit.)
• Presentation: brown powder
• Isoelectric Point: 7.2(Lit.)
• Optimum pH: 7.0(Lit.)
• Unit definition: amount of enzyme causing the production of 1mg of purpurogallin in 20 seconds at 20°C

RZ: ≥0.6 RZ Definition: The ratio of the absorbance at 403nm to the absorbance at 275nm. This value is an expression of the ratio of hemin to protein content.

Specifications

• Color: Brown
• pH: 7
• For Use With (Application): ELISA, immunoblotting, immunohistochemistry
• Content And Storage: -20°C
• Source: Horseradish
• Quantity: 50 kU
• Product Type: Peroxidase
• Molecular Weight (g/mol): 44000
• Inhibitors: p-Aminobenzoic Acid, Azide, Cyclopropanone, L-cystine, Dichromate, Ethylenethiourea, Hydroxylamine, Sulfite and Vanadate and Divalent Metals Cd, Co, Cu, Fe, Mn, Ni, Pb
• Form: Lyophilized Powder