MT-ATP6, Mouse anti-Human, Clone: 1G7-1G2, Millipore Sigma™

Catalog No.	Quantity
MABS199525	25 μg

Catalog No. MABS199525 Supplier MilliporeSigma Supplier No. MABS199525UG

Description

Mouse Monoclonal Antibody

Anti-MT-ATP6, clone 1G7-1G2, Cat. No. MABS1995, is a mouse monoclonal antibody that detects ATP synthase subunit a and has been tested for use in Western Blotting.

ATP synthase subunit a (UniProt: P00846; also known as F-ATPase protein 6) is encoded by the MT-ATP6 (also known as ATP6, ATPASE6, MTATP6) gene (Gene ID: 4508) in human. F-ATPase Protein 6 is an inner mitochondrial membrane protein and is a component of Complex V, which produces ATP from ADP in the presence of proton gradient across the membrane. F-type ATPases have 2 components, CF1 - the catalytic core - and CF0- the membrane proton channel. The CF1 catalytic core contains five subunits: alpha3, beta3, gamma1, delta1, epsilon1 and CF0 has three main subunits known as a, b, and c. Together they form a rotary motor. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Defects in MT-ATP6 gene cause multiple mitochondrial diseases, such as Leber hereditary optic neuropathy (LHON), which is characterized by acute and subacute loss of central vision due to optic nerve dysfunction. Some defects also lead to mitochondrial complex V deficiency that leads to neuropathy, ataxia, and hypertrophic cardiomyopathy.

Specifications

• Antigen: MT-ATP6
• Applications: Western Blot
• Classification: Monoclonal
• Clone: 1G7-1G2
• Conjugate: Unconjugated
• Dilution: Western Blotting Analysis: 0.5μg/mL from a representative lot detected MT-ATP6 in mitochondria from human neonatal dermal fibroblasts and mitochondria from human neonatal dermal fibroblasts depleted of mtDNA (Courtesy of Michael F. Marusich, Ph.D., mAbDx, Inc., Eugene, OR USA).
• Formulation: Purified mouse monoclonal antibody IgG2b in HEPES-Buffered Saline (15 mM HEPES, 150 mM NaCl,  pH 7.2) with 0.02% sodium azide.
• Gene Alias: ATP synthase subunit a;F-ATPase protein 6
• Gene Symbols: MT-ATP6;ATP6;ATPASE6;MTATP6
• Host Species: Mouse
• Immunogen: A synthetic peptide corresponding to the N-terminus of human MT-ATP6.
• Purification Method: Protein L
• Quantity: 25 μg
• Regulatory Status: RUO
• Research Discipline: Signaling
• Primary or Secondary: Primary
• Test Specificity: Clone 1G7-1G2 detects ATP synthase subunit a (MT-ATP6) in mitochondria isolated from human cells.
• Target Species: Human
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Form: Purified
• Isotype: IgG2b κ