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Invitrogen™ Calpain 2 Monoclonal Antibody (107-82)
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Catalog No. MA3942
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100 μg
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Catalog No. Quantity
MA3942 100 μg
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Catalog No. MA3942 Supplier Invitrogen™ Supplier No. MA3942
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Description

Mouse Monoclonal Antibody

MA3-942 detects the 80 kDa subunit of m-calpain in human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, rat myoblasts, kidney, liver and spleen, pig cultured cells, and rabbit samples. This antibody does not cross-react with mu-calpain, n-calpain, calmodulin or calpastatin. MA3-942 has been successfully used in Western blot, IF, immunohistochemistry, and ELISA procedures. By Western blot, this antibody detects an ~80 kDa protein representing m-calpain in bovine platelets. Immunocytochemical staining of m-calpain in LLC-PK1 cells with MA3-942 results in diffuse cytoplasmic staining. This product has not been shown to be effective in immunoprecipitation experiments. The MA3-942 antigen is purified bovine skeletal muscle 80 kDa subunit of m-calpain. This antibody recognizes an epitope between amino acids 502-699 (domain III/IV) of human calpain.

Mu-Calpain, also known as Calpain-I, and m-calpain, also known as Calpain-II, are intracellular, calcium-dependent cysteine proteases. Mu-calpain has a micromolar sensitivity (thus the mu-) as compared to the millimolar calcium sensitivity of m-calpain.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Calpain 2
Applications ELISA, Flow Cytometry, Immunohistochemistry (Frozen), Western Blot, Immunocytochemistry
Classification Monoclonal
Clone 107-82
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 1mg/mL BSA and 0.05% sodium azide
Gene CAPN2
Gene Accession No. P06814, P17655, P43367, Q07009, Q27971
Gene Alias 80 kDa M-calpain subunit; AI326419; Calcium-activated neutral proteinase 2; calcium-dependent protease; CALP80; calpain 2; calpain 2, (m/II) large subunit; calpain 2, large [catalytic] subunit; calpain 2, large subunit; calpain II; calpain large polypeptide L2; Calpain M-type; calpain, large polypeptide L2; calpain-2 catalytic subunit; Calpain-2 large subunit; CANP 2; CANP2; CANPL2; CANPml; Capa2; Capa-2; CAPN2; cysteine proteinase; m-calpain; m-calpain 80 kDa large subunit; m-calpain large subunit; m-calpin; mCANP; millimolar-calpain
Gene Symbols CAPN2
Host Species Mouse
Immunogen Purified bovine skeletal muscle 80 kDa subunit of m-calpain.
Purification Method Protein A
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 100009092, 281662, 29154, 397393, 824
Target Species Human, Rat, Rabbit, Bovine, Pig
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Product Type Antibody
Form Liquid
Isotype IgG1
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