Invitrogen™ Calpain 2 Monoclonal Antibody (107-82)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA3942

Catalog No. MA3942

Description

MA3-942 detects the 80 kDa subunit of m-calpain in human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, rat myoblasts, kidney, liver and spleen, pig cultured cells, and rabbit samples. This antibody does not cross-react with mu-calpain, n-calpain, calmodulin or calpastatin. MA3-942 has been successfully used in Western blot, IF, immunohistochemistry, and ELISA procedures. By Western blot, this antibody detects an ~80 kDa protein representing m-calpain in bovine platelets. Immunocytochemical staining of m-calpain in LLC-PK1 cells with MA3-942 results in diffuse cytoplasmic staining. This product has not been shown to be effective in immunoprecipitation experiments. The MA3-942 antigen is purified bovine skeletal muscle 80 kDa subunit of m-calpain. This antibody recognizes an epitope between amino acids 502-699 (domain III/IV) of human calpain.

Mu-Calpain, also known as Calpain-I, and m-calpain, also known as Calpain-II, are intracellular, calcium-dependent cysteine proteases. Mu-calpain has a micromolar sensitivity (thus the mu-) as compared to the millimolar calcium sensitivity of m-calpain.

Specifications

• Antigen: Calpain 2
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: P06814, P17655, P43367, Q07009, Q27971
• Gene Symbols: CAPN2
• Immunogen: Purified bovine skeletal muscle 80 kDa subunit of m-calpain.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Rat, Rabbit, Bovine, Pig
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Frozen), Western Blot, Immunocytochemistry
• Clone: 107-82
• Conjugate: Unconjugated
• Gene: CAPN2
• Gene Alias: 80 kDa M-calpain subunit; AI326419; Calcium-activated neutral proteinase 2; calcium-dependent protease; CALP80; calpain 2; calpain 2, (m/II) large subunit; calpain 2, large [catalytic] subunit; calpain 2, large subunit; calpain II; calpain large polypeptide L2; Calpain M-type; calpain, large polypeptide L2; calpain-2 catalytic subunit; Calpain-2 large subunit; CANP 2; CANP2; CANPL2; CANPml; Capa2; Capa-2; CAPN2; cysteine proteinase; m-calpain; m-calpain 80 kDa large subunit; m-calpain large subunit; m-calpin; mCANP; millimolar-calpain
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 100009092, 281662, 29154, 397393, 824
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid