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Thermo Scientific™ Muller-Kauffmann Tetrathionate Broth Base (Dehydrated)

Catalog No. CM0343B
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CM0343B 500 g
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Description

Isolate salmonellae and suppress the Proteus species with this medium.

Isolate salmonellae and suppress the Proteus species with Thermo Scientific™ Oxoid™ Muller-Kauffmann Tetrathionate Broth Base (Dehydrated). The enrichment medium contains brilliant green and ox bile to suppress the commensal organisms, thus improving the isolation of salmonellae.

Muller1 developed this medium in 1923. It was later modified by Kauffmann2,3 with the addition of brilliant green and ox bile to suppress commensal organisms and thus improve the isolation of salmonellae.

The brilliant green dye used in the medium has been shown to be critical and Chroma or BDH brands should be used. It is essential that the dye is added as directed because heating the brilliant green or attempting to incorporate it in the basal medium seriously impairs its selective action.
The addition of novobiocin at 40mg per litre of broth was described by Jeffries4 to suppress the growth of Proteus species.

The addition of novobiocin at 40mg per litre of broth was described by Jeffries4 to suppress the growth of Proteus species.

Muller-Kauffmann Tetrathionate Broth should not be used if Salmonella typhi is suspected.

Muller-Kauffmann Tetrathionate Broth was used in a large-scale investigation between nine laboratories in eight different countries5.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand

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Specifications

Certifications/Compliance Industrial Reference: NMKL.
Description Muller-Kauffmann Tetrathionate Broth Base
Form Powder
Product Type Dehydrated Culture Media
Quantity 500 g
Yield For 6.1L medium
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