Invitrogen™ MultiShot™ BL21 Star™ (DE3) Chemically Competent E. coli Cells, 96-tube rack

Catalog No.	Quantity
A40007057	1 x 96 Tubes (50 μL/Well)

Catalog No. A40007057 Supplier Invitrogen™ Supplier No. A40007057

Description

MultiShot BL21 Star (DE3) Chemically Competent E. coli Cells deliver enhanced mRNA stability for high-yield protein expression. The flexible 96-tube rack format supports scalable, efficient workflows for cloning and expression experiments.

Invitrogen MultiShot BL21 Star (DE3) Chemically Competent E. coli Cells are designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7 promoter-based expression systems. These cells are engineered to carry the λDE3 lysogen that contains the T7 RNA polymerase gene under control of the lacUV5 promoter and pre-aliquoted in a rack of 96 tubes for convenience and ease of use with as few as one reaction but also enabling use in a semi-automated workflow.

Features of MultiShot BL21 Star (DE3) competent cells include:

• High transformation efficiency: >1x10⁷ cfu/μg pUC19 DNA
• Specifically constructed for high-level expression of recombinant proteins
• Compatible with automated liquid handling platforms
• Transform a flexible number from 1 to 96 tubes at the time
• Competent cells pre-aliquoted into a rack of 96 tubes, ready for transformation

MultiShot BL21 Star (DE3) competent cells in 96-tube rack format can be used flexibly used flexibly in any number from 1 to 96 tubes for low to medium throughput needs. The BL21 (DE3) E. coli strain is one of the most popular host strains used to produce recombinant proteins and adapted in research and industrial applications. IPTG is required to induce expression of the T7 RNA polymerase cascade system that in turn transcribes the T7 promoter regulated target gene.

The BL21(DE3) strain is derived from the E. coli B strain that does not contain the lon protease and is also deficient in the outer membrane protease OmpT. The lack of two key proteases reduces degradation of heterologous proteins expressed in the strains. Additionally, several other strain features make them especially suited for protein production, namely fast growth in minimal medium and a lower acetate production when grown in high glucose.

MultiShot BL21 Star (DE3) competent cells offer enhanced mRNA stability due to a mutation in the RNaseE gene (rne131) that reduces levels of endogenous RNases and mRNA degradation, thereby increasing the stability of mRNA transcripts and increasing protein yield. The BL21 Star strain has higher basal expression of heterologous genes than BL21 (DE3) strains, due to the increased stability of mRNA. Thus, strains such as BL21-AI are recommended for toxic protein expression.

Genotype

F^–ompT hsdSB (r_B^–, m_B^–) gal dcm rne131 (DE3)

Specifications

• Product Type: Chemically Competent Cells
• Includes: pUC19 DNA, S.O.C media
• Transformation Efficiency Level: ≥1.0 x 10⁷
• Shipping Condition: Dry Ice
• Species: E. coli
• Format: Tube Rack
• Product Line: MultiShot
• For Use With (Application): Protein Expression
• Quantity: 1 x 96 Tubes (50 μL/Well)

For Research Use Only.