Invitrogen™ MultiShot™ DH5α™ T1R Chemically Competent E. coli Cells, 96-tube rack

Catalog No.	Quantity
A40007060	1 x 96 Tubes (50 μL/Well)

Catalog No. A40007060 Supplier Invitrogen™ Supplier No. A40007060

Description

MultiShot DH5α T1R Chemically Competent E. coli Cells provide high-efficiency cloning with flexible workflow capacity. The convenient 96-tube rack format supports scalable DNA manipulation and plasmid propagation in high-throughput applications.

Invitrogen MultiShot DH5α T1R Chemically Competent E. coli Cells are a well-known, versatile strain that is used in many everyday cloning applications.

Features of DH5α T1R competent cells include:

• TOP10 strain ideal for high-efficiency cloning and plasmid propagation
• Microtiter plate format design convenient for automated high-throughput cloning
• mcrA mutation that supports efficient transfection of methylated DNA
• lacZΔM15 allele that permits rapid blue/white screening of transfectants
• endA1 mutation that helps reduce endonuclease activity and improve plasmid DNA yields
• recA1 mutation that helps reduce unwanted recombination

MultiShot DH5α T1R competent cells are prepared by a proprietary modification of the Hanahan method. These cells are suitable for use in high efficiency transformations in applications where a low amount of DNA is used or to construct libraries using plasmid-derived vectors. In addition to supporting blue/white screening, recA1 and endA1 mutations in DH5α cells increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

The Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors and can be used for blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal. DH5α cells are capable of being transformed efficiently with large plasmids and can also serve as a host for the M13mp cloning vectors if a lawn of DH5αF'IQ (or similar) is provided to allow plaque formation.

Genotype

F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1

Specifications

• Product Type: Chemically Competent Cells
• Includes: pUC19 DNA, S.O.C media
• Transformation Efficiency Level: ≥1.0 x 10⁸
• Shipping Condition: Dry Ice
• Species: E. coli
• Format: Tube Rack
• Product Line: MultiShot
• For Use With (Application): Cloning
• Quantity: 1 x 96 Tubes (50 μL/Well)

For Research Use Only.