Invitrogen™ MultiShot™ FlexPlate DH5α T1^R Competent Cells

Description

Includes

Box 1:

• MultiShot™ FlexPlate DH5α™ T1R Chemically Competent E. coli
• One 96-well plate, 20 μl per well

Box 2:

• pUC19 DNA (10 pg/μl): 1 vial, 50 μl
• S.O.C. medium: 2 bottles, 10 ml each

MultiShot FlexPlate DH5α T1^R Chemically Competent E. coli cells are cloning competent cells derived from a well-known, versatile DH5α strain that can be used in many everyday cloning applications.

MultiShot FlexPlate DH5α T1^R Chemically Competent E. coli cells are cloning competent cells derived from a well-known, versatile DH5α strain that can be used in many everyday cloning applications. These cells are pre-aliquoted in a 96-well PCR plate to increase transformation productivity, and the FlexPlate format can be divided down to any combination of 8-well segments for flexibility in transformation throughput.

All of our T1^R strains have the tonA (also known as fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries.

Reliable DH5α T1^R cloning capabilities
MultiShot FlexPlate DH5α T1^R competent E. coli cells are able to achieve transformation efficiencies of >1 x 10⁸ cfu/μg control DNA. This strain is recommended for transformations of cloned unmethylated DNA, routine cloning of genes into large plasmids, and is suitable for difficult clone constructions, such as blunt-end ligations or cloning using limited amount of DNA.

The DH5α T1^R strain carries the hsdR mutation to prevent restriction of foreign DNA and the endA1 mutation that increases the yield of plasmid DNA prepared using miniprep protocols. The lacZΔM15 marker provides α-complementation of the ß-galactosidase gene allowing blue-white screening on agar plates containing X-Gal or Bluo-Gal.

MultiShot FlexPlate DH5α T1^R Competent Cells offer:
• Transformation efficiency of >1 x 10⁸ transformants/μg plasmid DNA
• tonA (fhuA) genotype to confer resistance to T1 and T5 phage
• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• lacZΔM15 marker for blue-white screening
• endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I
• recA1 for reduced occurrence of non-specific recombination in cloned DNA

Increase transformation productivity with MultiShot FlexPlate format
The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into 8-well segments for flexibility in transformation throughput. Addition of DNA to the MultiShot FlexPlate Competent Cells can be quickly performed with a multichannel pipet or a liquid handling system. After addition of DNA, the cells can be efficiently transformed by heat-shock using a water bath, heat block, or thermal cycler.

Key benefits of the MultiShot FlexPlate format
• Increased throughput—compatible with automated liquid handling platforms
• Flexible—transform in increments of 8 wells to entire 96-well plate
• Convenient—competent cells pre-aliquoted into 96-well plate, ready for transformation

Genotype
F^– φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (r_K–, m_K+) phoA supE44 λ–thi-1 gyrA96 relA1 tonA

Find the strain and format that fits your needs
• Many other E. coli strains and formats of chemically competent and electrocompetent cells are also available to meet specific transformation needs.
• For high-throughput transformation, choose from our collection of MultiShot formatted comp cells.
• We also offer bacterial growth media in various formats.

Specifications

• Product Type: Chemically Competent Cells
• Contains F' Episome: No
• Cloning Methylated DNA: No
• Content And Storage: • MultiShot FlexPlate DH5α T1^R Competent Cells (1 x 96-well plate at 20 μL/well)
  Store at –80°C.
  
  • pUC19 DNA (50 μL at 10 pg/μL)
  Store at –20°C.
  
  • S.O.C. Medium (2 x 10 mL)
  Store at 4°C or room temperature.
  
  
• Antibiotic Resistance Bacterial: No
• Blue/White Screening: Yes (lacZΔM15)
• High-throughput Compatibility: High
• Preparing Unmethylated DNA: No
• Reduces Recombination: Yes (recA1)
• Shipping Condition: Dry Ice
• T1 Phage - Resistant (tonA): Yes
• Species: E. coli (K12)
• Format: 96-well Plate
• Product Line: Multishot™
• Quantity: 1 x 96 well plate (20 μl/well)

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.